Comparison of functions between CYP24A1and Vitamin D3 through Microarray and pathway analysis

Abstract

CYP24A1 is a rate-limiting enzyme in the metabolic process of vitamin D, which hydroxylate 25 (OH) D3,1,25(OH)2D3 into 24,25(OH)2D3 and 1α,24,25(OH) 3D3 respectively. After microarray analysis of gene set GSE12449, we found CYP24A1 participate in three important pathways, Focal adhesion, ECM-receptor interaction and antigen process. Vitamin D3 make a significance in several gene ontology process, such as basement membrane, response to wounding, positive regulation of gene expression, iron ion binding, integrin binding and so on. In comparison, CYP24A1 also make a role in several process, such as regulation of apoptotic process, transmembrane transport,fructose metabolic process and lipid transport,defense response to virus function and so on. These results reveal the functional commons and differences between CYP24A1 and Vitamin D3, which may indicate the next step study directions between these two genes.