Determination of thiram in tobacco by surface-enhanced differential Raman spectroscopy

Abstract

This study had developed a rapid, simple, accurate, and efficient method for the qualitative and quantitative determination of thiram residues tobacco and tobacco products based on surface-enhanced Raman spectroscopy and differential Raman spectroscopy. Acetonitrile was used as the extractant and PSA and GCB as the scavengers to effectively reduce the matrix effect. At the same time, sodium chloride and hydrochloric acid were used to induce the agglomeration of the target molecule and the nano particles of enhanced colloid substrates. With this approach to improve the sensitivity of analysis, our results showed that the fine linear equations of thiram with the correlation coefficients equaled 0.9986. The detection of limits was 0.0084 mg/L and the recovery was within 85–117% and the RSD was less than 10%. The detection method of thiram had a good accuracy and precision, which was potentially suitable for other dithoicarbamate pesticide residues in tobacco and tobacco products.